VitroJet

How do you benefit?

Our scope is to help the researcher optimize sample preparation workflows. By providing a controllable automated environment, you can determine the settings for your particle of interest.

We are not just about vitrification, we are about workflow optimization.

Automation

The entire sample preparation workflow is integrated within the VitroJet, including the plasma treatment of the sample carrier. Pre-clipped autogrids are processed automatically without user interference. Therefore, many manual transfer steps can be avoided, reducing the risk of transfer induced contamination.

Relevant parameters in the sample preparation process can be set, adjusted, and stored. By giving you control on the workflow through automation, high reproducibility can be obtained.

Controlled sample deposition

Thin layer deposition is controlled via a unique pin printing technology only using up to 1nL of sample. Adjustment of parameters, such as writing velocity and distance between pin and grid, allow for tuning of the layer thickness. Dew point control of the sample ensures minimal evaporation. With the controlled pin printing technology, there is no need for blotting anymore, reducing potential damage to either sample or carrier.

Superior cooling rates

Jet vitrification cools the sample starting in the center of the grid, ensuring vitrification of the sample first rather than the grid. Furthermore, it enables vitrification of higher thermal masses due to the high cooling rates. 

VitroJet- Optical Inspection cryo-EM atlas

Visual Inspection

The sample deposition process is visualized in real-time with a high resolution camera. The early visual inspection of the grid allows for evaluation of grid quality during sample preparation. Valuable microscope screening time can be reduced so that you can focus on the best grids.

Superior

cooling rates

Picoliter

volume deposition

Pre-clipped

autogrid vitrification

Frequently Asked Questions

What grids can be used?

Basically, we can process all grids that allow pre-clipping in Autogrid carrier rings. When depositing sample, the writing pin does not touch the actual grid, so there is no physical contact between pin and grid. To date, we have not encountered damage of grids due to shear force when depositing sample.

Can we modify settings for plasma treatment?

The plasma treatment of grids is integrated to ensure reproducibility (timings, type of treatment, etc.) between grids. However, if you prefer to treat your grids in a specific way, the step inside the VitroJet can be bypassed. The VitroJet will be equipped with a plasma cleaner, allowing for the use of different gases.

How many grids can we produce in a single session?

The VitroJet is equipped with a cassettes holding up to 12 pre-clipped grids. Normally, this is the maximum number of grids used in a single session. Of course, if you place another storage cassette in the VitroJet after the first one is finished, you can process as many as you like, and operation is limited by the contamination of your cryogenic compartment.

How long does the nitrogen/ethane last?

The VitroJet is equipped with a system that automatically refills both liquid nitrogen and ethane. The degree of contamination determines how long you can work with the system. Use in a low-humidity environment is preferred.

Are we restricted to a type or brand of microscope?

The VitroJet is designed for optimal control and reproducibility, therefore pre-clipped grids are used because they allow automated handling. Although pre-clipped grids are used, (side-entry) cryoholders can be adjusted with only minor changes to fit pre-clipped grids. Contact CryoSol for further information about compatibility with your microscope or cryoholder.

Are we restricted to only one sample per session?

No, you’re not. You can change your sample at any point in the session. This applies to any other parameters the user wishes to adjust in the session. 

Where can we place the VitroJet?

The VitroJet is designed as a bench-top device, it has a footprint of approximately 80 x 80 cm / 32 x 32 inch. Although the VitroJet is a closed system, ideally the environment has a relative low humidity and limited flow, to ensure minimal ice contamination. 

What are the requirements with respect to connections to the VitroJet?

The VitroJet is designed to operate at 230 VAC/50 Hz and/or 110 VAC/60 Hz. A dry nitrogen gas connection is required for drying. Depending on your plasma cleaning requirements, you may want to connect other cleaning gases. Distilled water is used for obtaining the humidity in the sample deposition module.    

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